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Promega passive lysis 1x buffer e1941
Passive Lysis 1x Buffer E1941, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/passive+lysis+buffer/passive+lysis+buffer/pmc12163403-82-15-20
Average 90 stars, based on 1 article reviews
passive lysis 1x buffer e1941 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title:
Article Snippet: Twenty-four hours after infection, the cells were transfected using 1 ng/μl of poly(I:C) and Xtremegene 9 (Sigma-Aldrich) transfection reagent in DMEM per manufacturer’s instructions. .. Twenty-four hours after transfection, the media was aspirated and 50 μl of 1× passive lysis buffer (Promega) was added. ..

Article Title:
Article Snippet: The secondary structure of the RNA transcripts was predicted using the mfold program.61 Analysis of miAng-mediated luciferase knockdown Huh-7 cells were co-transfected in P24-well plates in triplicate with 50 ng LucAngA or LucAngB and 50 ng or 250 ng of each miAng/ miSCR plasmid using Lipofectamine 3000 reagent (Thermo Fisher Scientific). .. Transfected cells were collected 48 h post-transfection in passive lysis buffer (Promega, Thermo Fisher Scientific), and FL and RL activities were measured in cell lysates using the Dual-Luciferase Reporter Assay System (Promega, Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

Article Title: Supporting Information
Article Snippet: .. Twenty-four h later, pGL3pNfe2l2 was transfected for 5 additional h. Forty-eight hours after the transfection of pGL3-pNfe2l2, cells were lysed with Passive Lysis Buffer (Promega Corp.). .. Levels of luciferase and βgalactosidase activity were assayed 48 h later with Luciferase Assay System kit (Promega Corp.) and Luminiscent β-galactosidase Detection kit II (Clontech, Takara Bio Inc., Shiga, Japan), respectively.

Lysis:

Article Title:
Article Snippet: Twenty-four hours after infection, the cells were transfected using 1 ng/μl of poly(I:C) and Xtremegene 9 (Sigma-Aldrich) transfection reagent in DMEM per manufacturer’s instructions. .. Twenty-four hours after transfection, the media was aspirated and 50 μl of 1× passive lysis buffer (Promega) was added. ..

Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions
Article Snippet: STARR luciferase validation vector bearing CMV enhancer (Addgene #99312) (Muerdter et al. 2018) was used to monitor transfection efficiency. .. 48 hrs post-transfection, cells were treated with 10 nM of E2 or 100% ethanol (vehicle control) for 24 h prior to harvest using 50 μL of 1× passive lysis buffer (Promega) per well. .. 20 μL lysate was used for each assay with the Dual-Luciferase® Reporter Assay kit (Promega) according to the manufacturer’s instructions using the M200Pro TECAN Luminometer in technical duplicate with three biological replicates.

Article Title: Supporting Information for The helicase-like transcription factor redirects the autophagic flux and restricts human T cell leukemia virus type 1 infection
Article Snippet: .. After twenty-four hours of co-culture, cells were harvested and lysed with passive lysis buffer (Promega) for 5 min at 4°C. ..

Article Title:
Article Snippet: The secondary structure of the RNA transcripts was predicted using the mfold program.61 Analysis of miAng-mediated luciferase knockdown Huh-7 cells were co-transfected in P24-well plates in triplicate with 50 ng LucAngA or LucAngB and 50 ng or 250 ng of each miAng/ miSCR plasmid using Lipofectamine 3000 reagent (Thermo Fisher Scientific). .. Transfected cells were collected 48 h post-transfection in passive lysis buffer (Promega, Thermo Fisher Scientific), and FL and RL activities were measured in cell lysates using the Dual-Luciferase Reporter Assay System (Promega, Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

Article Title:
Article Snippet: The total amount of DNA was kept constant by using the appropriate empty vector. .. 145 After 24 hours post-transfection, cell extracts were generated using Passive Lysis Buffer 146 (Promega), and the extracts were assayed for firefly luciferase and Renilla luciferase activity using 147 the Dual-Luciferase® Reporter Assay system (Promega). .. Luminescence was measured using 148 the Glomax microplate luminometer (Promega).

Article Title: Supporting Information
Article Snippet: .. Twenty-four h later, pGL3pNfe2l2 was transfected for 5 additional h. Forty-eight hours after the transfection of pGL3-pNfe2l2, cells were lysed with Passive Lysis Buffer (Promega Corp.). .. Levels of luciferase and βgalactosidase activity were assayed 48 h later with Luciferase Assay System kit (Promega Corp.) and Luminiscent β-galactosidase Detection kit II (Clontech, Takara Bio Inc., Shiga, Japan), respectively.

Article Title: Supporting Information for The helicase-like transcription factor redirects the autophagic flux and restricts human T cell leukemia virus type 1 infection
Article Snippet: Hek293T cells at 80% confluency in 6 well-plates were transfected with 2μg of HLTF-N1, HLTF-N2, Tax-N1 and Tax-N2 mixed with 6μg polyethylenimine (PEI; Sigma-Aldrich) in 200μl of OPTI-MEM (Thermo Fisher). .. Twenty-four hours post-transfection, cells were lysed with passive lysis buffer (Promega) and luciferase activity was quantified with the Renilla substrate of the Dual-Luciferase Reporter Assay System (Promega) and a Berthold Lumat LB 9507 luminometer. .. RNA was extracted with the Nucleospin RNA Plus kit (Macherey Nagel) and reverse transcribed into cDNA using FastGene Scriptase II (Nippon Genetics) and random hexamers.

Control:

Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions
Article Snippet: STARR luciferase validation vector bearing CMV enhancer (Addgene #99312) (Muerdter et al. 2018) was used to monitor transfection efficiency. .. 48 hrs post-transfection, cells were treated with 10 nM of E2 or 100% ethanol (vehicle control) for 24 h prior to harvest using 50 μL of 1× passive lysis buffer (Promega) per well. .. 20 μL lysate was used for each assay with the Dual-Luciferase® Reporter Assay kit (Promega) according to the manufacturer’s instructions using the M200Pro TECAN Luminometer in technical duplicate with three biological replicates.

Reporter Assay:

Article Title:
Article Snippet: The secondary structure of the RNA transcripts was predicted using the mfold program.61 Analysis of miAng-mediated luciferase knockdown Huh-7 cells were co-transfected in P24-well plates in triplicate with 50 ng LucAngA or LucAngB and 50 ng or 250 ng of each miAng/ miSCR plasmid using Lipofectamine 3000 reagent (Thermo Fisher Scientific). .. Transfected cells were collected 48 h post-transfection in passive lysis buffer (Promega, Thermo Fisher Scientific), and FL and RL activities were measured in cell lysates using the Dual-Luciferase Reporter Assay System (Promega, Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

Article Title:
Article Snippet: The total amount of DNA was kept constant by using the appropriate empty vector. .. 145 After 24 hours post-transfection, cell extracts were generated using Passive Lysis Buffer 146 (Promega), and the extracts were assayed for firefly luciferase and Renilla luciferase activity using 147 the Dual-Luciferase® Reporter Assay system (Promega). .. Luminescence was measured using 148 the Glomax microplate luminometer (Promega).

Article Title: Supporting Information for The helicase-like transcription factor redirects the autophagic flux and restricts human T cell leukemia virus type 1 infection
Article Snippet: Hek293T cells at 80% confluency in 6 well-plates were transfected with 2μg of HLTF-N1, HLTF-N2, Tax-N1 and Tax-N2 mixed with 6μg polyethylenimine (PEI; Sigma-Aldrich) in 200μl of OPTI-MEM (Thermo Fisher). .. Twenty-four hours post-transfection, cells were lysed with passive lysis buffer (Promega) and luciferase activity was quantified with the Renilla substrate of the Dual-Luciferase Reporter Assay System (Promega) and a Berthold Lumat LB 9507 luminometer. .. RNA was extracted with the Nucleospin RNA Plus kit (Macherey Nagel) and reverse transcribed into cDNA using FastGene Scriptase II (Nippon Genetics) and random hexamers.

Generated:

Article Title:
Article Snippet: The total amount of DNA was kept constant by using the appropriate empty vector. .. 145 After 24 hours post-transfection, cell extracts were generated using Passive Lysis Buffer 146 (Promega), and the extracts were assayed for firefly luciferase and Renilla luciferase activity using 147 the Dual-Luciferase® Reporter Assay system (Promega). .. Luminescence was measured using 148 the Glomax microplate luminometer (Promega).

Luciferase:

Article Title:
Article Snippet: The total amount of DNA was kept constant by using the appropriate empty vector. .. 145 After 24 hours post-transfection, cell extracts were generated using Passive Lysis Buffer 146 (Promega), and the extracts were assayed for firefly luciferase and Renilla luciferase activity using 147 the Dual-Luciferase® Reporter Assay system (Promega). .. Luminescence was measured using 148 the Glomax microplate luminometer (Promega).

Article Title: Supporting Information for The helicase-like transcription factor redirects the autophagic flux and restricts human T cell leukemia virus type 1 infection
Article Snippet: Hek293T cells at 80% confluency in 6 well-plates were transfected with 2μg of HLTF-N1, HLTF-N2, Tax-N1 and Tax-N2 mixed with 6μg polyethylenimine (PEI; Sigma-Aldrich) in 200μl of OPTI-MEM (Thermo Fisher). .. Twenty-four hours post-transfection, cells were lysed with passive lysis buffer (Promega) and luciferase activity was quantified with the Renilla substrate of the Dual-Luciferase Reporter Assay System (Promega) and a Berthold Lumat LB 9507 luminometer. .. RNA was extracted with the Nucleospin RNA Plus kit (Macherey Nagel) and reverse transcribed into cDNA using FastGene Scriptase II (Nippon Genetics) and random hexamers.

Activity Assay:

Article Title:
Article Snippet: The total amount of DNA was kept constant by using the appropriate empty vector. .. 145 After 24 hours post-transfection, cell extracts were generated using Passive Lysis Buffer 146 (Promega), and the extracts were assayed for firefly luciferase and Renilla luciferase activity using 147 the Dual-Luciferase® Reporter Assay system (Promega). .. Luminescence was measured using 148 the Glomax microplate luminometer (Promega).

Article Title: Supporting Information for The helicase-like transcription factor redirects the autophagic flux and restricts human T cell leukemia virus type 1 infection
Article Snippet: Hek293T cells at 80% confluency in 6 well-plates were transfected with 2μg of HLTF-N1, HLTF-N2, Tax-N1 and Tax-N2 mixed with 6μg polyethylenimine (PEI; Sigma-Aldrich) in 200μl of OPTI-MEM (Thermo Fisher). .. Twenty-four hours post-transfection, cells were lysed with passive lysis buffer (Promega) and luciferase activity was quantified with the Renilla substrate of the Dual-Luciferase Reporter Assay System (Promega) and a Berthold Lumat LB 9507 luminometer. .. RNA was extracted with the Nucleospin RNA Plus kit (Macherey Nagel) and reverse transcribed into cDNA using FastGene Scriptase II (Nippon Genetics) and random hexamers.

other:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: The pellet generated was then resuspended in 80 ml Peptide Lysis Buffer (25 mM HEPES pH 7.4, 0.1× Passive Lysis Buffer (Promega Corporation), 1 ml/ml lysozyme and 0.03 U/μl RQ1 DNase (Promega Corporation)) and incubated at room temperature for 15 minutes.



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